Britain's Shaun McKeown claimed Paralympic silver in the men's C3 3km individual pursuit at the Velodrome as Joseph Berenyi of the United States won gold.
Defending champion Darren Kenny bounced back from his qualification disappointment to claim bronze in a world record of 3:35.257 - faster than the gold medal-winning time.
Fellow Brits Aileen McGlynn and Helen Scott earlier won silver in the women's blind and visually impaired time-trial at the London 2012 velodrome as Australia won gold.
McGlynn and Scott clocked one minute 09.469 seconds, but Australia's Felicity Johnson and Stephanie Morton were last on the track and stole victory by 0.550secs in a Paralympic record of 1min 08.919secs.
It was the first time McGlynn has tasted Paralympic defeat in the event after the 39-year-old from Glasgow won gold in Athens and Beijing with Ellen Hunter as pilot. McGlynn and Scott finished second to the Australian pair at the World Championships earlier this year and were runners-up once more.
New Zealand's Phillipa Gray and Laura Thompson claimed bronze in 1:11.245, with Britain's Lora Turnham and Fiona Duncan fourth in 1:11.479.
Mark Colbourne qualified fastest in the men's C1 individual pursuit in a world record of 3:53.970.
Colbourne finished second to Li Zhang Yu in the C1-2-3 one-kilometre time-trial on day one and watched the Chinese rider set a world record of 4:00.235 in 3km pursuit qualifying before improving the mark himself.
The 42-year-old Welshman was set to meet Li in the race for gold on Friday afternoon. In the men's C3 3km individual pursuit, 2008 champion Darren Kenny can win bronze at best after qualifying in 3:37.977, but team-mate Shaun McKeown reached the gold medal ride-off.
McKeown was one of three riders to dip beneath Kenny's world record set in Beijing, advancing in second place in 3:36.427. Joseph Berenyi of the United States set a world record of 3:36.148 in the first heat and will meet McKeown in the final.