Wigan have chosen to play reigning Super League champions Leeds in Friday's semi-final at the DW Stadium.
The Warriors had the right to pick their opponents under the controversial Clubcall rule and opted to avoid Challenge Cup winners Warrington, the only other choice open to them, when they announced their decision at Sunday lunchtime.
The double-chasing Wolves, therefore, will play St Helens in the second semi-final at Langtree Park on Saturday.
Warrington, who beat Hull 24-12 in Saturday's preliminary semi-final, suffered a 28-6 home defeat by Saints in the opening round of the play-offs and as a consequence lost home advantage at the last-four stage.
Leeds, who were chosen by Warrington in last year's semi-finals, are aiming to win the title once more from fifth place and have started to look in ominous form with impressive wins over Wakefield and Catalan Dragons so far.
Table-toppers Wigan have done the double over the Rhinos this year and will have the chance to avenge their surprise 39-28 Challenge Cup semi-final defeat by Brian McDermott's men, who went on to lose to Warrington at Wembley.
Leeds coach Brian McDermott says his side's Cup win will count for little on Friday. He said: "It may count more for Wigan in terms of motivation but we won't read much into it.
"They beat us twice in the league and it wasn't a great day for us at home when they put 50 points past us."
Wigan coach Shaun Wane says his team will be out to avenge their Challenge Cup defeat and added: "We're still hurting from that performance in the cup. It was not so much the loss but we under-performed that day.
"I've been upset ever since. It is a game I'm desperate to put right."